EVALUASI NILAI HOMA-Β PADA TIKUS DIABETES TERINDUKSI STREPTOZOTOCIN SETELAH PEMBERIAN FERMENTASI TEH HIJAU
Keywords:
Diabetes mellitus, Kombucha, Pangan Fungsional, HOMA-β
Abstract
Hiperglikemia kronis berkontribusi terhadap gangguan fungsi sel β pankreas melalui peningkatan stres oksidatif dan disfungsi sekresi insulin. Kombucha merupakan minuman hasil fermentasi teh oleh kultur simbiotik bakteri dan ragi (SCOBY) yang diketahui mengandung berbagai senyawa bioaktif dengan potensi metabolik. Penelitian ini mengevaluasi efek pemberian kombucha teh hijau terhadap regulasi glukosa darah dan fungsi sel β pankreas pada model tikus diabetes yang diinduksi streptozotocin (STZ). Lima belas tikus Wistar jantan dibagi menjadi tiga kelompok, yaitu kelompok diabetes tanpa intervensi serta dua kelompok intervensi dengan dosis 1,25 mL/kgBB dan 2,5 mL/kgBB selama 14 hari. Pemberian kombucha menunjukkan penurunan kadar glukosa darah puasa serta perbaikan indeks HOMA-B secara signifikan dibandingkan kelompok diabetes. Dosis 2,5 mL/kgBB memberikan respons paling optimal. Temuan ini mengindikasikan bahwa kombucha teh hijau berpotensi mendukung perbaikan homeostasis glukosa melalui modulasi fungsi sel β pancreas.
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28. Fushimi T, Tayama K, Fukaya M, et al. The efficacy of acetic acid for glycogen repletion in rat skeletal muscle after exercise. Int J Sports Med. 2002;23(3):218-222. doi:10.1055/s-2002-23172
29. Fushimi T, Sato Y. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br J Nutr. 2005;94(5):714-719. doi:10.1079/bjn20051545
30. Sakakibara S, Yamauchi T, Oshima Y, Tsukamoto Y, Kadowaki T. Acetic acid activates hepatic AMPK and reduces hyperglycemia in diabetic KK-A(y) mice. Biochem Biophys Res Commun. 2006;344(2):597-604. doi:10.1016/j.bbrc.2006.03.176
2. IHME. Diabetes mellitus - level 3 cause. Published online 2020:2.
3. Rahmad Darmawan, Ira Humairah, Ema Qunianingsih, Ervi A. Munthe, Linda. Effect of Annona muricata L. (Soursop) on Blood Glucose Level in a Diabetic Rat Model: A Meta-Analysis. Trop J Nat Prod Res. 2025;9(9):4148-4154. doi:10.26538/tjnpr/v9i9.11
4. Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes. 2015;6(3):456. doi:10.4239/wjd.v6.i3.456
5. Eguchi N, Vaziri ND, Dafoe DC, Ichii H. The Role of Oxidative Stress in Pancreatic β Cell Dysfunction in Diabetes. Published online 2021.
6. Yedjou CG, Grigsby J, Mbemi A, et al. The Management of Diabetes Mellitus Using Medicinal Plants and Vitamins. Int J Mol Sci. 2023;24(10). doi:10.3390/ijms24109085
7. Chaudhury A, Duvoor C, Reddy Dendi VS, et al. Clinical Review of Antidiabetic Drugs: Implications for Type 2 Diabetes Mellitus Management. Front Endocrinol (Lausanne). 2017;8(January). doi:10.3389/fendo.2017.00006
8. Roglic G. WHO Global report on diabetes: A summary. Int J Noncommunicable Dis. 2016;1(1):3. doi:10.4103/2468-8827.184853
9. Onsun B, Toprak K, Sanlier N. Kombucha Tea : A Functional Beverage and All its Aspects. Published online 2025. doi:10.1007/s13668-025-00658-9
10. Antolak H, Piechota D, Kucharska A. Kombucha tea—A double power of bioactive compounds from tea and symbiotic culture of bacteria and yeasts (SCOBY). Antioxidants. 2021;10(10). doi:10.3390/antiox10101541
11. Jakubczyk K, Kupnicka P, Melkis K, et al. Effects of Fermentation Time and Type of Tea on the Content of Micronutrients in Kombucha Fermented Tea. Nutrients. 2022;14(22). doi:10.3390/nu14224828
12. Vázquez-Cabral BD, Larrosa-Pérez M, Gallegos-Infante JA, et al. Oak kombucha protects against oxidative stress and inflammatory processes. Chem Biol Interact. 2017;272:1-9. doi:10.1016/j.cbi.2017.05.001
13. Dias FO, K CS. Protective Effect of Kombucha on Diabetic Nephropathy in Streptozotocin - Induced Diabetic Rats. Int J Sci Res. 2016;5(3):945-948. doi:10.21275/v5i3.nov161951
14. Prasetia H, Setiawan AAR, Bardant TB, et al. STUDI POLA KONSUMSI TEH DI INDONESIA UNTUK MENDUKUNG DIVERSIFIKASI PRODUK YANG BERKELANJUTAN (A Study of Tea Consumption Pattern in Indonesia Toward Sustainable Product Diversification). Biopropal Ind. 2020;11(2):107. doi:10.36974/jbi.v11i2.6249
15. Rathi MH, Abdulhay HS. Total phenolic contents and antioxidant activity of extracts of tea (Black, green and white). Asian J Microbiol Biotechnol Environ Sci. 2018;20(February):S45-S49.
16. Xu S, Wang Y, Wang J, Geng W. Kombucha Reduces Hyperglycemia in Type 2 Diabetes of Mice by Regulating Gut Microbiota and Its Metabolites. Foods (Basel, Switzerland). 2022;11(5). doi:10.3390/foods11050754
17. Kaewkod T, Bovonsombut S, Tragoolpua Y. Efficacy of kombucha obtained from green, oolongand black teas on inhibition of pathogenic bacteria, antioxidation, and toxicity on colorectal cancer cell line. Microorganisms. 2019;7(12). doi:10.3390/microorganisms7120700
18. Yamauchi K, Sato Y, Nakasone Y, Aizawa T. Comparison of HOMA-IR , HOMA- β % and disposition index between US white men and Japanese men in Japan in the ERA JUMP study : was the calculation of disposition index legitimate ? Published online 2015:1679-1680. doi:10.1007/s00125-015-3612-x
19. Elsner M, Guldbakke B, Tiedge M, Munday R, Lenzen S. Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin. Diabetologia. 2000;43(12):1528-1533. doi:10.1007/s001250051564
20. Eleazu CO, Eleazu KC, Chukwuma S, Essien UN. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. J Diabetes Metab Disord. 2013;12(1):1-7. doi:10.1186/2251-6581-12-60
21. Zubaidah E, Ifadah RA, Kalsum U, et al. Anti-diabetes activity of Kombucha prepared from different snake fruit cultivars. Nutr Food Sci. 2019;49(2):333-343. doi:10.1108/NFS-07-2018-0201
22. Jakubczyk K, Kałduńska J, Kochman J, Janda K. Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants. 2020;9(5). doi:10.3390/antiox9050447
23. Wen L, Wu D, Tan X, et al. The Role of Catechins in Regulating Diabetes: An Update Review. Nutrients. 2022;14(21). doi:10.3390/nu14214681
24. Sun L, Miao M. Dietary polyphenols modulate starch digestion and glycaemic level: a review. Crit Rev Food Sci Nutr. 2020;60(4):541-555. doi:10.1080/10408398.2018.1544883
25. Man Z, Feng Y, Xiao J, Yang H, Wu X. Structural changes and molecular mechanism study on the inhibitory activity of epigallocatechin against α-glucosidase and α-amylase. Front Nutr. 2022;9(November):1-11. doi:10.3389/fnut.2022.948027
26. Kim YA, Keogh JB, Clifton PM. Polyphenols and glycémie control. Nutrients. 2016;8(1). doi:10.3390/nu8010017
27. Mitrou P, Petsiou E, Papakonstantinou E, et al. Vinegar Consumption Increases Insulin-Stimulated Glucose Uptake by the Forearm Muscle in Humans with Type 2 Diabetes. J Diabetes Res. 2015;2015. doi:10.1155/2015/175204
28. Fushimi T, Tayama K, Fukaya M, et al. The efficacy of acetic acid for glycogen repletion in rat skeletal muscle after exercise. Int J Sports Med. 2002;23(3):218-222. doi:10.1055/s-2002-23172
29. Fushimi T, Sato Y. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br J Nutr. 2005;94(5):714-719. doi:10.1079/bjn20051545
30. Sakakibara S, Yamauchi T, Oshima Y, Tsukamoto Y, Kadowaki T. Acetic acid activates hepatic AMPK and reduces hyperglycemia in diabetic KK-A(y) mice. Biochem Biophys Res Commun. 2006;344(2):597-604. doi:10.1016/j.bbrc.2006.03.176
Published
2026-06-01
Section
Artikel Penelitian
Copyright (c) 2026 Rahmad Darmawan
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